DNASU Next Generation Sequencing • 480.965.6795 | Email

About the DNASU Next Generation Sequencing Core

The Genomics Core at ASU offers next-generation sequencing services on the Illumina MiSeq and NextSeq 500 instruments, as well as a variety of library preparation services for a range of sample types and research applications.

Illumina NGS uses the concept of "sequencing by synthesis" to generate data, imaging each base as the DNA strand is being built. Because each strand (or more precisely, each cluster of identical strands) can be imaged independently, the sample does not need to be homogeneous. Instead of obtaining one long sequence beginning from a known priming site, this process results in many shorter sequences that can then be assembled into a genome, transcriptome, exome, or amplicon library, depending on the sample type. These shorter sequences are known as "reads."

The two sequencers available in the Core provide data output ranging from 1 million reads (for a Nano kit on the MiSeq) to 400 million reads (for a High Output kit on the NextSeq 500), in several different read lengths. This breadth of range enables us to sequence small proof-of-concept projects, large genome assembly projects, and everything in between.

If you are interested in our assistance with sample preparation, we offer several workflows for different sample types and applications. These primarily fall into three categories: RNA sequencing, targeted resequencing, and standard DNA sequencing. If you have genomic DNA, plasmid DNA, or PCR-purified DNA, we can add the Illumina adapters necessary for sequencing. We can screen your samples against a known set of genes using targeted amplicon panels, select specifically for exome regions and UTRs, and amplify out 16S regions for population genetics (in partnership with the ASU Microbiome Core). In addition, we offer a robust total RNA sequencing pipeline that can work with degraded RNA or RNA from FFPE samples, as well as a stranded mRNA sequencing pipeline.

Feel free to explore our website to gain a deeper understanding for NGS science and our technical services at the Genomics Core, and don't hesitate to contact us if you have any questions!