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Template Concentration

  For Plasmid DNA For purified PCR products
Template Concentration 50 ng/uL 3ng/ul per 100bp
Volume 10ul per well 10ul
Primer Concentration 1.6pmol/ul 0.4pmol/ul
Volume 10ul 10ul

For example, a concentration of 25ng/uL is appropriate for a 500bp PCR product.


Sequencing Primers

The following primers can be added to your reaction at your request for no additional charge.

Primer Name Primer Sequence
M13 Forward 5'-GTAAAACGACGGCCAGT-3'
M13 Reverse 5'-CAGGAAACAGCTATGACC-3'
T7 5'-AATACGACTCACTATAG-3'
T7 Terminator 5'-TATGCTAGTTATTGCTCAG-3'
SP6 5'-CATACGATTTAGGTGACACTATAG-3'
T3 5'-AAATTAACCCTCACTAAAGG-3'