Next Generation Sequencing

Next Generation Sequencing

The DNASU Sequencing facility offers Next Generation DNA Sequencing using the Illumina HiSeq 2000 platforms. This supports experimental applications such as high throughput DNA sequencing, genome wide association studies (GWAS), gene regulation analysis, SNP discovery and functional variation analysis, cytogenetic analysis, DNA-protein interaction analysis (ChIP-Seq), and sequencing-based methylation analysis.

Sanger Sequencing

Sequencing Core

The DNA sequencing services at Center for Personalized Diagnostics (CPD) provides investigators access to automated DNA sequencing for purified DNA and glycerol stocks. The facility accepts both individual samples and 96-well plates.

DNASU Plasmid repository

Plasmid Repository

DNASU is a central repository for plasmid clones and collections. We currently store and distribute over 191,000 plasmids including over 75,000 human and mouse plasmids, full genome collections from numerous organisms, the protein expression plasmids from the Protein Structure Initiative as the PSI:Biology-Materials Repository (PSI:Biology-MR), and both small and large collections from individual researchers.

NAPPA Protein Array Core

NAPPA Protein Array Core

The NAPPA Protein Array Core uses a novel protein microarray technology, called Nucleic Acid-Programmable Protein Array (NAPPA), which replaces the complex process of spotting purified proteins with the simple process of spotting plasmid DNA. Our facility offers high throughput DNA preparation, pre-set and custom protein array production, and array screening services.